C8

C8 columns have various applications in liquid chromatography (HPLC and UHPLC), especially when a less retentive stationary phase than C18 is desired or when working with highly nonpolar samples.

Main applications:

• Separation of moderately nonpolar compounds: small lipophilic molecules where intermediate retention is desirable.

• Analysis of biological and pharmaceutical samples: metabolites, drugs, and related substances that require lower retention to avoid long elution times.

• Separation of weak acids and bases: when lower retention than C18 is needed to prevent peak broadening or long equilibration times.

• Protein and peptide chromatography: in methods where reduced nonspecific interaction with the stationary phase is desired to maintain sample conformation and stability.

• Separation of extraction products and plastic/cosmetic options:** hydrophobic compounds that require an 8-carbon chain to balance retention.

• Rapid method development and scaling: in Design of Experiments (DoE) workflows to compare C8 with C18 and decide which provides better resolution and analysis times.

• Separation of steroids and light lipids: some analytes with intermediate polarity benefit from the lower retention of C8 compared to C18.

• Methods under specific pH conditions: when pH conditions may deprotect or destabilize interactions with the phase, C8 columns can offer good stability under acidic pH.

Octylsilane (C8) Columns

HPLC (High Performance Liquid Chromatography) columns are the heart of the high-resolution liquid chromatography system.

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